Antler fly
At least two big “fly announcements” are coming soon. Meanwhile, I am posting this photo of an antler fly (a type of fruit fly from New Guinea) for your entertainment.
Coming soon – big news for the fly obsessed
Author Archives: Brian Brown
More microfraction miracles
I call these “miracles” not in the religious sense, but in the unlikelihood that I would notice them in a “normal” sample with all the macro-garbage obscuring them.
The first is a male, perhaps of the genus Metopina, with the thick costal vein almost as long as the wing.
Metopina group male
Next is a bizarre female termitoxeniine that has not yet shed its wings.
female termitoxeniine
A relatively “normal” wingless female Chonocephalus.
female Chonocephalus
Finally, a flattened male of a new phorine genus with a short costa.
new phorine genus
And I still have many more vials to look through! Life is good.
All photos were made by Inna-Marie Strazhnik, who is a superb artist.
Microfractions rule!
I am sorting through a bunch of Malaise trap samples from Thailand from which all the insects larger than about 2 mm have been removed. The absence of larger insects makes all the tiny ones stand out, so these samples, the “microfraction” are golden. They are full of treasures: usually overlooked tiny things like Chonocephalus, termitoxeniines, and weird Metopina group males. I’ll publish more photos soon, but here are a couple I photographed previously.
a Metopina group female
new phorine genus
Note the shieldlike crest on the back of the head in the second photo. Totally bizarre.
Microfractions represent yet another largely unexplored frontier of tropical phorid diversity. After nearly 30 years of doing this, I can still be amazed and awed by “my” flies.
Problems photographing flies. 4. What type of light.
Here are some options; look at the abdomen:
popup flash - the worst
Significant “speckling” here, where each bristle reflects the flash. Ugly.
external flash on camera
flash with softbox diffuser
natural light
The natural light is so much better. Look at the details visible (click on the image to see a larger version). Too bad it is impractical.
I could go on and on about this, but even I’m getting bored. Besides, someone else has a great website about macrophotograpy lighting. Look at this site for amazing images and how they were made: http://orionmystery.blogspot.com/ . I don’t have to reinvent the wheel here.
Back to flies next time.
Problems photographing flies. 3. You need light.
Okay, based on parts 1 and 2 of this series, you want to use high apertures (lens f-stop settings) to get lots in focus, but you need to use intermediate apertures to avoid diffraction blurriness. Either you have to focus stack (often impractical in the field) or accept a compromise f-stop like f11. So how does f11 work for you?
In all but the brightest light, f11 (or f8) will require long exposure times, giving ample opportunity for you or the fly to move, blurring the exposure. We’re back to either needing a motionless fly (unlikely) or, this time, more light.
Wait, can’t you just dial up the ISO (sensor sensitivity) on your new digital camera so you can use a faster shutter speed? Yes, but you increase the digital graininess (“noise”) in the photo, such that resolution at high magnifications is destroyed.
Here are a series of closeups of bristles on a tachinid fly showing this effect:
ISO 100
ISO 200
ISO 400
ISO 800
ISO 1000
ISO 1600
ISO 6400
This series of shots tells me that, for my camera, ISO 200 is about the same as 100. For ISO 400-800 I get some degradation, but it is still pretty good. Above ISO 800, thing get pretty mushy. You need to check this in your camera, too.
The result is that changing ISO only helps me a little. If I want the highest quality images, I need more light. How to get this light is my next topic.
Why so spiny?
I got a lot of questions about why yesterday’s fly would be so spiny. I can think of two plausible answers, both of which might be right.
Firstly, such spiny flies are almost invariably found in species associated with ants, especially army ants. As evidence for this, here are 3 flies from the New World tropics found with Labidus army ants: Acontistoptera, in which the long setae (bristles) are found almost only on the wing rudiments, Adelopteromyia, which are spiny on the wing and on the body (especially the head), and Xanionotum, which has multiple rows transversely across the abdomen.
The large setae could be used to fend off attacking ants, like a porcupine, or for sensory purposes in the darkness of underground ant colonies. Or both. One thing to keep in mind is that the flies probably can move the setae, erecting them or laying them down. They are much more flexible, mobile, and speedy than you might think, as they literally runs circles around the host ants.
A brief respite from photography posts: another bizarre phorid
My friend in New Zealand, Hugh Oliver, saw the picture of the wingless female phorid in my last blog post, and asked for more photos of weird phorids. I didn’t even know he was looking at my blog, but just for him I am posting this photo of an extremely bizarre specimen we found just this week in material from Thailand. I think it is a female of the genus Rhynchomicropteron, but if so, it is an extremely unusual one! Thanks to Lisa Gonzalez for pointing it out to me, and Inna-Marie Strazhnik for photographing it. Maybe it can be number 16 in Terry Wheeler’s posts about why flies are great.
Problems photographing flies. 2. To stack or not to stack?
female Aenigmatias
As I showed in the last post, at high magnifications you can only have a small amount of your subject in the sharpest focus because diffraction limits useable depth of field. Now you have two choices: stacking or settling for some different compromise.
The image above is a blended stack of 25 images, all of which only have a bit of the fly in focus. I used a program called Zerene Stacker to do this, but there are other options. You can make some phenomenal images this way (just look at the forums at www.photomacrography.net), especially using tens to hundreds of source images, but there is a catch – your subjects have to be motionless, preferably dead. Some field photographers can get short stacks of a few images of resting flies, but generally flies are 2. active. For me, it is better to pick a compromise lens setting like f 8-11, as discussed last time, to get good shots of lively, moving flies. If you want more information on stacking, though, see the free resources at www.macrostop.com.
Next: how do you get enough light to use f 11?
Problems photographing flies. 1. Of what is your camera capable?
A tachinid fly - looks good, right?
Flies are fantastic subjects for photography, but they present many challenges because they are
1. small,
2.active,
3. often metallic and bristly.
All of these are factors that cause problems that I’ll discuss in the next few postings.
[The reason I’m writing about this is that there is so little good macrophotography information about insects like flies. I assume interested readers know the basics of 35 mm digital photography. If this subject doesn’t grab you, go back and re-read the last post by Lisa Gonzalez, which was a real crowd-pleaser]
When you photograph small things at high magnification, your depth of field is frighteningly shallow. Take a shot at f 1.4 and you’ll get maybe one tarsomere and a wingtip in focus. The rest will be a blur. Therefore, you need to close down to f 22 or f 32 to get everything in focus- correct? Unfortunately, when you do this, you start to distort the image through diffraction. It’s not really a problem when photographing big things (like landscapes), but flies are 1. small.
Above is a good-sized tachinid fly. I photographed it with my camera (Nikon D-7000) on a tripod, using a 125 mm macro lens set at f 22. At this magnification it looks pretty sharp, but look closer (below). You’ll softness from diffraction that no fly obsessed photographer (and I hope you are one) would tolerate. And since most flies are smaller than this tachinid, what we see in this magnified view is ALL we’ll get.
f 22 -blech!
At the next setting, f 16, sharpness is definitely better. Scroll down to see other settings.
f 16, a little better
f 11, better yet
f8 - sharp
f 5.6 - who knew?
The problem, of course, is that by f 5.6 the legs are all out of focus. You have to pick a compromise (f 11 works for me) or do something else, photostacking, which only works well with stationary objects (and remember, flies are 2. active). More compromises are ahead.
To get the best results, you first need to know what your camera can do. Everyone needs to test their camera and lenses this way to see what is possible. Then you can move on too what is practical.
Next- how do you get to use that f 11 setting?
Keep your eyes on the flies
[This is a guest post by Lisa Gonzalez, one of my field team members.]
Lisa Gonzalez in demonstration mode
Last week I had the incredible honor of joining a small team that included Wendy, a parataxonomist; Inna, a scientific illustrator and videographer; and Kat, an accomplished amateur photographer; under the guidance of the respected Dipterist Dr. Brian Brown, to the lush lowland tropics of Costa Rica on a phorid fly foraging expedition. Our goal was to record and observe parasitic phorid fly behavior– in particular to begin unraveling the mysteries surrounding certain phorid flies in the genera Apocephalus and Dohrniphora— as well as collecting any specimens that struck our interest.
My first trip to this beautiful, magical country was 10 years ago, but every day that has gone by since has been a day that I have anticipated returning. So suffice it to say I was beyond excited at the prospect of spending entire days under the forest canopy, scanning the ground and leaves for parasitic phorids and their specific hosts.
People who know me know that my passion for insects runs deep, but I still get an occasional incredulous glance, a slightly furrowed brow or a polite smile and shrug from my non-entomologist friends as I explain that the vast majority of our trip will be dedicated to looking for 3 mm sized flies (even though they know it is part of a scientific project, as in, WORK.) No zip lines, no sipping mango margaritas on the beach –sugar on the rim, hold the salt!– no waiting for a quetzal to fly by for that perfect photo op.
No, my friends, we will be looking for flies, cool parasitic ones, because they are out there, lurking in the jungle, waiting for someone to observe them doing things that are infinitely more fascinating to me than the showiest toucan or the noisiest oropendola. Somebody needs to give them the attention they deserve!
After a morning cup of that fine, fine Costa Rican coffee, we used the help of the phorid flies themselves, namely their incredible ability to detect the alarm pheromones of their hosts. We searched the tree trunks and undergrowth for the specific types of ants that serve as the hosts (Odontomachus sp. and Paraponera clavata in this case) so that we could collect them, crush them, and thus attract the flies we so anxiously sought, a technique that Brian serendipitously but no less ingeniously discovered 20 years ago.
As the flies started to hone in on the chemicals released by the distressed ants, I felt myself becoming completely entranced by the miniature drama unfolding at my feet. This is the point where I struggle to find my mental middle zone, maintaining a focus on the flies while not becoming too tunnel-visioned; still being aware of my surroundings since, after all, we are in a jungle with stinging ants and wasps everywhere, and an occasional venomous snake, but most importantly, not wanting to miss any phorid action on the sidelines.
Keeping your eyes on the flies and not being distracted by bedazzling morphos or adorable sloths (my goodness, they ARE cute!) reaps great rewards for anyone with even a modest interest in animal biology and biodiversity. For an entomologist, or those willing to scale down their perspective and peer into the microcosmos, it is a truly awesome experience to see a minute female fly risk her own life by attacking an ant at least twice her size, desperately needing a place to lay her egg (or eggs), using a variety of bodily tools, such as ovipositors shaped like sabers or mouthparts serrated like knives. These females are so alert and nimble, like little ninjas, and although the outcome of their efforts, which results in decapitation or an “‘Alien’ chest burster” style host death depending on the phorid species, may seem cruel to our human sensibilities, I see a brave and resilient mother struggling to care for her young (that’s a whole lot of anthropormorphism for one sentence, I know!)
Brian decided to expand our efforts in the field on this trip by collecting female phorids and hosts and observing them in captivity in our make shift lab. He very generously put me in charge of this part of the project, proclaiming that I am “good at keeping bugs alive” from my years spent as the museum’s insect zoo technician, a professional extension of the top secret insectary I kept in my closet when I still lived with my parents many years ago. After several days of working out humidity issues, adding extra masking tape for the sneaky escape artists, and trying to provide a stress free environment for the flies to “go about their business,” we were able to observe some provocative behavior in captivity. Some preliminary work was done with the species Dohrniphora conlanorum, and we successfully reared larvae of Apocephalus paraponerae. I was alone in the lab when I first spied the chubby little bundles of maggot-y joy in the abdominal cavity of the Paraponera ants, and in my solitude was able to get up and do my celebratory maggot dance without shame. The pure excitement over seeing a world that most people rarely stop to notice, the thrill of something new on the other end of your scope, is greater than a million zip line adrenaline rushes or brain-in-a-jacuzzi delicious tropical rum buzzes to me. I am so elated to have been a part of this project, and I hope you enjoyed my attempt to briefly describe that elation. Now to start counting the days until I return once more…